Informatics Publishing Limited

Monika A. Rana ¹, Hasumati A. Raj ²

Affiliations
1 Department of Quality Assurance, Shree Dhanvantary Pharmacy College, Kim Surat, Gujarat, IN
2 Department of Quality Assurance, Shree Dhanvantary Pharmacy College, Kim Surat, Gujarat

Abstract

A simple, accurate and precise spectroscopic method was developed for Levosulpiride and 2-methoxy 5- sulfamoylbenzoyl benzoic acid methyl ester in synthetic mixture using Q absorbance Ratio Method. In this spectroscopic method, 231.50 nm (as an iso-absorptive point) and 240.60 nm wavelengths (λmax of any of the two drugs) were selected for measurement of absorptivity. Both the drugs show linearity in a concentration range of 10-30 μg/ml at their respective λmax and at the isoabsorptive point. The relative standard deviation for accuracy, precision studies were found to be within the acceptance range (<2%). The limit of determination was 0.084μg/ml and 0.110μg/ml for Levosulpiride and 2-methoxy 5-sulfamoylbenzoyl benzoic acid methyl ester, respectively. The limit of quantification was 0.257μg/ml and 0.330 μg/ml for Levosulpiride and2-methoxy 5-sulfamoylbenzoyl benzoic acid methyl ester, respectively. Recovery of Levosulpiride and 2-methoxy 5-sulfamoylbenzoyl benzoic acid methyl ester were found to be 101.12 % and 101.89 % respectively confirming the accuracy of the proposed method. The proposed method is recommended for routine analysis since they are rapid, simple, accurate and also sensitive and specific by no heating and no organic solvent extraction.

Keywords

Levosulpiride, 2-Methoxy 5-Sulfamoylbenzoyl Benzoic Acid Methyl Ester, Q Absorbance Ratio Method.

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Monika A. Rana ¹, Hasumati A. Raj ¹

Affiliations
1 Department of Quality Assurance, Shree Dhanvantary Pharmacy College, Kim Surat, Gujarat, IN

Abstract

A simple, accurate and precise chromatography method was developed for Levosulpiride and 2-methoxy 5- sulfamoylbenzoyl benzoic acid methyl ester in synthetic mixture using RP-HPLC Method. In this chromatography method, both drug show its peak at 232 nm. Both the drugs show linearity in a concentration range of 10-50 μg/ml at their respective λmax. Optimize Mobile Phase was Water : Methanol : Acetonitrile (70 : 15 : 15). The relative standard deviation for accuracy, precision studies were found to be within the acceptance range (<2%). The limit of determination was 0.14μg/ml and 0.19μg/ml for Levosulpiride and 2-methoxy 5- sulfamoylbenzoyl benzoic acid methyl ester, respectively. The limit of quantification was 0.42μg/ml and 0.59μg/ml for Levosulpiride and 2-methoxy 5-sulfamoylbenzoyl benzoic acid methyl ester, respectively. Recovery of Levosulpiride and 2-methoxy 5-sulfamoylbenzoyl benzoic acid methyl ester were found to be 100.16 % and 99.15 % respectively confirming the accuracy of the proposed method. The proposed method is recommended for routine analysis since they are rapid, simple, accurate and also sensitive and specific by no heating and no organic solvent extraction.

Keywords

Levosulpiride, 2-Methoxy 5-Sulfamoylbenzoyl Benzoic Acid Methyl Ester, RP-HPLC Method.

Full Text